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Human SCF Protein

CAT: 0013-GTR18984639-01Size: 2 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18984639-01Size:2 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant of Human SCF protein
Product Name Alternative
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Source
Sf9, Insect Cells
Purity
Greater than 98.0% as determined by analysis by SDS-PAGE.
Bioactivity
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions
Stability: Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics) . 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard
Preservative
The protein is supplied in 1xPBS, pH 7.4.

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