CAS9 Protein

CAT: 0013-GTR18984096-01Size: 50 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18984096-01Size:50 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease

Product Name Alternative

CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.

Source

Escherichia Coli

Field of Research

Molecular Biology

Purity

Greater than 90% as determined by SDS-PAGE.

Form

Sterile Filtered colorless solution.

Storage Conditions

Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles

Notes

For research use only.

Applications Notes

Enzymatic Activity: 97% of PCR product digestion after 30 minutes at 37°C. Endonuclease Activity: The incubation of 20pmol of CAS9 protein with 1μg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis. Non-specific DNAse activity: The incubation of 20pmol of CAS9 protein with 1μg of lambda DNA at 37°C for 4 hours is free of detectable DNA degradation, as visualized on agarose gel

Preservative

The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.

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