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Cas9 Antibody

CAT: 0013-GTR18187007Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18187007Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cas 9 antibody
Product Name Alternative
Rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody
UniProt
Q99ZW2
Reactivity
Bacteria
Immunogen
Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Target
Cas9
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Molecular Biology
Concentration
1.0 mg/mL
Dilution
ELISA: 1:25,000-1:30,000, IF: 1μg/mL, WB: 1μg/mL
Purity
Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IF, Multiplex Assay, WB
NCBI Accession Number
NP_269215.1
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

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