PCNA Recombinant Mouse Monoclonal Antibody [A6-G11-R]

PCNA Recombinant Mouse Monoclonal Antibody [A6-G11-R]

• Description: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
• Product Name Alternative: ATLD2 antibody cb16 antibody Cyclin antibody DNA polymerase delta auxiliary protein antibody etID36690.10 antibody fa28e03 antibody fb36g03 antibody HGCN8729 antibody MGC8367 antibody Mutagen-sensitive 209 protein antibody OTTHUMP00000030189 antibody OTTHUMP00000030190 antibody PCNA antibody Pcna/cyclin antibody PCNA_HUMAN antibody PCNAR antibody Polymerase delta accessory protein antibody Proliferating cell nuclear antigen antibody wu:fa28e03 antibody wu:fb36g05 antibody
• Abbreviation: Proliferating cell nuclear antigen PCNA Cyclin
• CAS Number: 9000-83-3
• Swiss Prot: P12004 Human, P17918 Mouse, P04961 Rat
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human PCNA aa 1-261.
• Isotype: IgG1
• Clone: A6-G11-R
• Conjugation: Non-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, IP
• Positive Control: HeLa cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, K-562 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, HeLa, Raji cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, human liver tissue lysate, HepG2, human lymph node tissue, human melanoma tissue, rat spleen tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000 ;IHC-P: 1:2,000 ;IF-Cell: 1:100-1:2,000 ;IP: 1-2μg/sample
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 29 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes