Anti-TCP1 beta/CCT2 Antibody Picoband®, HRP Conjugate

Anti-TCP1 beta/CCT2 Antibody Picoband®, HRP Conjugate

• Background: T-complex protein 1 subunit beta (TCP1 beta) is a protein that in humans is encoded by the CCT2 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
• Description: Boster Bio Anti-TCP1 beta/CCT2 Antibody Picoband® catalog # PB9992. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: T-complex protein 1 subunit beta;TCP-1-beta;CCT-beta;CCT2;99D8.1, CCTB;
• Gene Name: T-complex protein 1 subunit beta
• Gene ID: 10576
• UniProt: P78371
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human CCT2 recombinant protein (Position: E414-C535). Human CCT2 shares 95.9% and 93.4% amino acid (aa) sequence identity with mouse and rat CCT2, respectively.
• Clonality: Polyclonal
• Applications: Flow Cytometry,IF,IHC,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. .
• References & Citations: 1. "Entrez Gene: CCT2 chaperonin containing TCP1, subunit 2 (beta)". 2. Won KA, Schumacher RJ, Farr GW, Horwich AL, Reed SI (Dec 1998). "Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT". Molecular and Cellular Biology 18 (12): 7584–9.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 57488 MW
• Observed Molecular Weight: 57 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human