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TCP1 beta Antibody / CCT2

CAT: 0800-R32477Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32477Size:100 µg
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Description
T-complex protein 1 subunit beta (TCP1 beta) is a protein that in humans is encoded by the CCT2 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC) . This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P78371
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids E414-C535 from the human protein were used as the immunogen for the TCP1 beta antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
Prior to reconstitution, store at 4oC. After reconstitution, the TCP1 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TCP1 beta antibody is available for research use only.
Storage Conditions
Prior to reconstitution, store at 4°C. After reconstitution, the TCP1 beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the TCP1 beta antibody should be determined by the researcher.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of 1) rat brain, 2) rat spleen, 3) rat C6, 4) rat PC-12, 5) mouse brain, 6) mouse spleen, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.

UniProtKB · P78371

T-complex protein 1 subunit beta

TCPB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P78371
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCT2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TCP-1-beta
EC number
3.6.1.-
Processing
—
Secondary accessions
A8K402, B5BTY7, B7Z243, B7Z7K4, B7ZAT2, Q14D36, Q6IAT3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Molecular function

ChaperoneHydrolase

Cellular component

Cytoplasm
Product Datasheet
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