Anti-Replication factor C subunit 1 RFC1 Antibody Picoband®, Biotine Conjugate

Anti-Replication factor C subunit 1 RFC1 Antibody Picoband®, Biotine Conjugate

• Background: RFC (Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
• Description: Boster Bio Anti-Replication factor C subunit 1 RFC1 Antibody catalog # PA1716. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Replication factor C subunit 1;Activator 1 140 kDa subunit;A1 140 kDa subunit;Activator 1 large subunit;Activator 1 subunit 1;DNA-binding protein PO-GA;Replication factor C 140 kDa subunit;RF-C 140 kDa subunit;RFC140;Replication factor C large subunit;RFC1;RFC140;
• Gene Name: Replication factor C subunit 1
• UniProt: P35251
• Host: Rabbit
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1.
• Clonality: Polyclonal
• Applications: IF,IHC,ICC,WB,Flow Cytometry
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. .
• References & Citations: 1. Bermudez, V. P., Maniwa, Y., Tappin, I., Ozato, K., Yokomori, K., Hurwitz, J. The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA. Proc. Nat. Acad. Sci. 100: 10237-10242, 2003. 2. Peoples, R., Perez-Jurado, L., Wang, Y.-K., Kaplan, P., Francke, U. The gene for replication factor C subunit 2 (RFC2) is within the 7q11.23 Williams syndrome deletion. (Letter)Am. J. Hum. Genet. 58: 1370-1373, 1996.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 128255 MW
• Observed Molecular Weight: 150 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human