Anti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2), Biotine Conjugate

Anti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2), Biotine Conjugate

• Background: Erlin-2 is a protein that in humans is encoded by the ERLIN2 gene. This gene encodes a member of the SPFH domain-containing family of lipid raft-associated proteins. The encoded protein is localized to lipid rafts of the endoplasmic reticulum and plays a critical role in inositol 1,4,5-trisphosphate (IP3) signaling by mediating ER-associated degradation of activated IP3 receptors. Mutations in this gene are a cause of spastic paraplegia-18 (SPG18). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Description: Boster Bio Anti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) catalog # M07042-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5
• Gene Name: chaperonin containing TCP1 subunit 3
• Gene ID: 11160
• UniProt: O94905
• Host: Mouse
• Reactivity: Human
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human Erlin-2/ERLIN2 recombinant protein (Position: D87-N339).
• Clonality: Monoclonal
• Clone: Clone: 3H9A2
• Applications: WB,IHC,ICC,IF,Flow Cytometry
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
• References & Citations: 1. Al-Saif, A., Bohlega, S., Al-Mohanna, F. Loss of ERLIN2 function leads to juvenile primary lateral sclerosis. Ann. Neurol. 72: 510-516, 2012. 2. Alazami, A. M., Adly, N., Al Dhalaan, H., Alkuraya, F. S. A nullimorphic ERLIN2 mutation defines a complicated hereditary spastic paraplegia locus (SPG18). Neurogenetics 12: 333-336, 2011. 3. Browman, D. T., Resek, M. E., Zajchowski, L. D., Robbins, S. M. Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER. J. Cell Sci. 119: 3149-3160, 2006.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Observed Molecular Weight: 43 kDa
• Fragment: Mouse IgG2b
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human