Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2F10), Biotine Conjugate

Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2F10), Biotine Conjugate

• Background: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
• Description: Boster Bio Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2F10) catalog # M02659. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Arylsulfatase A; ASA; Cerebroside-sulfatase; Arylsulfatase A component B; Arylsulfatase A component C; ARSA
• Gene Name: arylsulfatase A
• Gene ID: 5158
• UniProt: P35913
• Host: Mouse
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).
• Clonality: Monoclonal
• Clone: Clone: 8I2F10
• Applications: WB,IHC,ICC,IF,Flow Cytometry
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Function: Hydrolyzes cerebroside sulfate.
• References & Citations: 1. Hmani-Aifa, M., Benzina, Z., Zulfiqar, F., Dhouib, H., Shahzadi, A., Ghorbel, A., Rebai, A., Soderkvist, P., Riazuddin, S., Kimberling, W. J., Ayadi, H. Identification of two new mutations in the GPR98 and the PDE6B genes segregating in a Tunisian family. Europ. J. Hum. Genet. 17: 474-482, 2009. 2. Tsang, S. H., Woodruff, M. L., Jun, L., Mahajan, V., Yamashita, C. K., Pedersen, R., Lin, C.-S., Goff, S. P., Rosenberg, T., Larsen, M., Farber, D. B., Nusinowitz, S. Transgenic mice carrying the H258N mutation in the gene encoding the beta-subunit of phosphodiesterase-6 (PDE6B) provide a model for human congenital stationary night blindness. Hum. Mutat. 28: 243-254, 2007. 3. Zhao, L., Zabel, M. K., Wang, X., Ma, W., Shah, P., Fariss, R. N., Qian, H., Parkhurst, C. N., Gan, W.-B., Wong, W. T. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Molec. Med. 7: 1179-1197, 2015.
• Storage Conditions: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Observed Molecular Weight: 98 kDa
• Fragment: Mouse IgG2b
• Applications Notes: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human