Anti-PDE6 alpha/PDE6A Antibody Picoband®-FITC Conjugate

Anti-PDE6 alpha/PDE6A Antibody Picoband®-FITC Conjugate

• Background: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
• Description: Boster Bio Anti-PDE6 alpha/PDE6A Antibody Picoband® catalog # A07800-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27
• Gene Name: SRY-box 15
• Gene ID: 5145
• UniProt: P16499
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human PDE6 alpha/PDE6A recombinant protein (Position: K11-R237).
• Clonality: Polyclonal
• Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Binds to the 5'-AACAAT-3' sequence.
• References & Citations: 1. Corton, M., Blanco, M. J., Torres, M., Sanchez-Salorio, M., Carracedo, A., Brion, M. Identification of a novel mutation in the human PDE6A gene in autosomal recessive retinitis pigmentosa: homology with the nmf28/nmf28 mice model. (Letter) Clin. Genet. 78: 495-498, 2010. 2. Khan, S. Y., Ali, S., Naeem, M. A., Khan, S. N., Husnain, T., Butt, N. H., Qazi, Z. A., Akram, J., Riazuddin, S., Ayyagari, R., Hejtmancik, J. F., Riazuddin, S. A. Splice-site mutations identified in PDE6A responsible for retinitis pigmentosa in consanguineous Pakistani families. Molec. Vision 21: 871-882, 2015. 3. Tuntivanich, N., Pittler, S. J., Fischer, A. J., Omar, G., Kiupel, M., Weber, A., Yao, S., Steibel, J. P., Khan, N. W., Petersen-Jones, S. M. Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation. Invest. Ophthal. Vis. Sci. 50: 801-813, 2009.
• Storage Conditions: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 49229 MW
• Observed Molecular Weight: 100 kDa
• Fragment: Rabbit IgG
• Applications Notes: Western blot, 0.1-0.25μg/ml,Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
  Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -