Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit

Human SIGLEC-1/CD169 PicoKine® Quick ELISA Kit

• Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
• Description: Human CD169/SIGLEC-1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human SIGLEC1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Sialoadhesin;Sialic acid-binding Ig-like lectin 1;Siglec-1;CD169;SIGLEC1;SN;
• Gene Name: Sialoadhesin
• Gene ID: 6614
• UniProt: Q9BZZ2
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S20-Q1641
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
• Specificity: Natural and recombinant human CD169
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	278	276	287	263	276	8.57	3.1%
  Sample 2	1821	2107	2081	1805	1953	140.91	7.2%
  Sample 3	4073	4478	4741	4142	4358	268.77	6.1%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 182624 MW
• Protein Name: Sialoadhesin