Mouse Integrin alpha 10 beta 1 (ITGA10&ITGB1) Heterodimer Protein, His Tag&Tag Free

• Background:

  Human integrin alpha (10) I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha (10) I did recognize collagen types I-VI and laminin-1 in a Mg (2+) -dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.

• Description:

  Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-M52W4) is expressed from human 293 cells (HEK293) . It contains AA Phe 23 - Thr 1118 (ITGA10) & Gln 21 - Asp 728 (ITGB1) (Accession # E9PXZ3-1 (ITGA10) & P09055-1 (ITGB1) ) .

• Specifications:

  Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA10 and ITGB1, has a calculated MW of 126.6 kDa (ITGA10) and 83.5 kDa (ITGB1) . Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGA10) and 100-130 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  E9PXZ3-1 (ITGA10) & P09055-1 (ITGB1)

• Expression Region:

  Phe 23 - Thr 1118 (ITGA10) & Gln 21 - Asp 728 (ITGB1)

• Host:

  HEK293

• Target:

  Integrin alpha 10 beta 1

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human Collagen IV at 2 μg/mL (100 μL/well) can bind Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W4) with a linear range of 0.039-1.25 μg/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  126.6 kDa (ITGA10) & 83.5 kDa (ITGB1)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  1mg*1

• Species:

  Mouse