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PDL1 Recombinant Protein

CAT: 0013-GTR18906728Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR18906728Size:0.1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PDL1 Recombinant Protein
Product Name Alternative
PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
UniProt
Q9NZQ7
Tag
His Tag
Source
HEK293 cells
Field of Research
Immunology & Inflammation
Purity
>98% as determined by SEC-HPLC and SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human PDL1 /CD274 Protein at 5μg/mL (100 μl/well), can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 0. 16-2. 4μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2μg/ml (100μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 0. 05-1μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0. 6-5μg/ml.
Form
Lyophilized
Molecular Weight
26 kDa
Storage Conditions
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This recombinant protein can be used for WB. For research use only.
Tested Applications
WB
NCBI Accession Number
NP_054862.1
Preservative
PBS, pH 7.4
AA Sequence
Phe 19 - Arg 238

UniProtKB · Q9NZQ7

Programmed cell death 1 ligand 1

PD1L1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NZQ7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD274
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1
EC number
—
Processing
Precursor
Secondary accessions
B2RBA2, B4DU27, Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneEndosomeMembraneNucleusSecreted

Disease

Diabetes mellitus

PTM

Disulfide bondGlycoproteinUbl conjugation

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

Receptor

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