Products for Research Use Only

PU.1 Antibody

CAT: 0013-GTR17697581Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697581Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to SPI1
Product Name Alternative
Anti spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody, anti hCG_25181 antibody, anti SFPI1 antibody, anti SPI-1 antibody, anti SPI-A antibody, anti 31 kDa transforming protein antibody
Reactivity
Human
Target
PU.1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DLYQRQTHEYY
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
31.2; 31.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275 (26) :19552-9.; PMID: 10867017) . Recommended concentration: 0.03-0.1 μg/ml.
Prediction Reactivity
Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6688

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