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PU.1 Polyclonal Antibody

CAT: 0749-ABP52285-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52285-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. Spi-1 proto-oncogene can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this SPI1.
Description
Rabbit Anti-PU.1 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
SPI1; Transcription factor PU.1; 31 kDa-transforming protein
Gene ID
6688
Accession Number
P17947
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Synthesized peptide derived from the Internal region of human PU.1 at AA range: 150-230
Clonality
Polyclonal
Tag
Transcription factor PU.1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
32kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
PU.1 Polyclonal Antibody detects endogenous levels of PU.1 protein.
Observed Molecular Weight
32
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.
Other Product Names
SPI1; Transcription factor PU.1; 31 kDa-transforming protein
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P17947
Isotype
Rabbit IgG

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