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ERN2/IRE1b Antibody

CAT: 0013-GTR17698101Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17698101Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to ERN2
Product Name Alternative
Anti ERN2 antibody, anti endoplasmic reticulum to nucleus signaling 2 antibody, anti IRE1b antibody, anti ER to nucleus signalling 2 antibody, anti IRE1 beta antibody, anti IRE1, S. cerevisiae, homolog of antibody, anti endoplasmic reticulum to nucleus signalling 2 antibody, anti inositol-requiring 1 beta antibody
Target
ERN2 / IRE1b
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QKRLQSPSKQAQPLD
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 0.3 μg/ml
Molecular Weight
107
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 37kDa band in Human Duodenum, Pancreas and Placenta lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_150296.3. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Human
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10595

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