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Recombinant Staphylococcus aureus Sortase A (srtA), partial

CAT: 0013-GTR18894088-02Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18894088-02Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Staphylococcus aureus Sortase A (srtA), partial spans the amino acid sequence from region 25-206aa. Purity: Greater than 85% as determined by SDS-PAGE.
Product Name Alternative
Surface protein sorting A
UniProt
Q2FV99
Expression Region
25-206aa
Tag
N-terminal 6xHis-tagged
Source
E.coli
Origin
Staphylococcus aureus (strain NCTC 8325)
Field of Research
Microbiology
Purity
Greater than 85% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Molecular Weight
24.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
AKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

UniProtKB · Q2FV99

Sortase A

SRTA_STAA8 · Staphylococcus aureus (strain NCTC 8325 / PS 47)

View on UniProt ↗
Primary accession
Q2FV99
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
srtA
Protein existence
1: Evidence at protein level
Organism
Staphylococcus aureus (strain NCTC 8325 / PS 47)
Taxonomy ID
93061
Alternative names
—
EC number
3.4.22.70
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Cellular component

Cell membraneMembrane

Molecular function

HydrolaseProteaseThiol protease

Ligand

Metal-binding

Domain

TransmembraneTransmembrane helix

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