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Monkey TNFA protein (Active)

CAT: 0013-GTR18881650-02Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18881650-02Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Monkey TNFA protein (Active) spans the amino acid sequence from region 77-233aa. Purity: > 95% as determined by SDS-PAGE and HPLC.
Product Name Alternative
Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, , NTF
UniProt
P48094
Expression Region
77-233aa
Tag
Tag-Free
Source
E.Coli
Origin
Macaca mulatta (Rhesus macaque)
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
> 95% as determined by SDS-PAGE and HPLC.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10 ^ 7 IU/mg in the presence of actinomycin D.
Form
Lyophilized powder
Molecular Weight
17.3 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm filtered PBS, pH 7.4, 5 % trehalose
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELTDNQLVVPSEGLYLIYSQVLFKGQGCPSNHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINLPDYLDFAESGQVYFGIIAL

UniProtKB · P48094

Tumor necrosis factor

TNFA_MACMU · Macaca mulatta

View on UniProt ↗
Primary accession
P48094
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNF
Protein existence
2: Evidence at transcript level
Organism
Macaca mulatta (Rhesus macaque)
Taxonomy ID
9544
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5TM21, Q8HZD6
Protein keywords

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinLipoproteinMyristatePhosphoprotein

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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