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Rat VEGF ELISA Kit

CAT: 0013-GTR18879068-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18879068-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Rat VEGF.
Product Name Alternative
VEGFA; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor
Reactivity
Rat
Field of Research
Cardiovascular Research; Cell Biology
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
8 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF in samples. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
15.6 pg/ml-1000 pg/ml

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