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Human β-NGF ELISA Kit

CAT: 0013-GTR18878713-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878713-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human β-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human β-NGF.
Product Name Alternative
Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide) ; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67
Reactivity
Human
Field of Research
Neuroscience
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
16 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human β-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate β-NGF in samples. An antibody specific for β-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any β-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for β-NGF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of β-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
31.25 pg/mL- 2000 pg/mL

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