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Human Leptin ELISA Kit

CAT: 0013-GTR18878691-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878691-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Human Leptin .
Product Name Alternative
FLJ94114; LEP; LEP_HUMAN; LEPD; Leptin; Leptin Precursor Obesity Factor; OB; Obese protein
Reactivity
Human
Field of Research
Metabolism Research
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
16 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human Leptin ELISA Kit employs a two-site sandwich ELISA to quantitate Leptin in samples. An antibody specific for Leptin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Leptin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Leptin is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
31.25 pg/mL- 2000 pg/mL

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