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Human Leptin (LEP) ELISA Kit

CAT: 0013-GTR18807570-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18807570-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin (LEP) .
Product Name Alternative
OB ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit, Obesity Factor ELISA Kit, Obese Protein ELISA Kit
UniProt
P41159
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.06 ng/mL
References & Citations
Jolanta Lis-Kuberka et al. Gestational diabetes mellitus–induced adipokine dysregulation and links to metabolic programming risks Frontiers in Endocrinology, (2026)
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · P41159

Leptin

LEP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41159
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LEP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O15158, Q56A88
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Disease

Diabetes mellitusDisease variantObesity

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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