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Pig Orexin (OX) ELISA Kit

CAT: 0013-GTR18876129-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18876129-01Size:48 Tests
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Description
Pig Orexin (OX) ELISA Kit
Product Name Alternative
HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor
UniProt
O77668
Reactivity
Porcine
Target
OX
Field of Research
Neuroscience
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
4.53 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig OX in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

UniProtKB · O77668

Hypocretin neuropeptide precursor

OREX_PIG · Sus scrofa

View on UniProt ↗
Primary accession
O77668
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HCRT
Protein existence
2: Evidence at transcript level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q000Y9, Q9TTA6
Protein keywords

PTM

AmidationCleavage on pair of basic residuesDisulfide bondPyrrolidone carboxylic acid

Cellular component

Cytoplasmic vesicleEndoplasmic reticulumSynapse

Molecular function

Neuropeptide

Technical term

Reference proteome

Domain

Signal

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