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Mouse KRT10 (Keratin 10) Microsample ELISA Kit

CAT: 0013-GTR18873554-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873554-01Size:48 Tests
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Description
Mouse KRT10 (Keratin 10) Microsample ELISA Kit
Product Name Alternative
K10; CK10; KPP; Cytokeratin 10; Epidermolytic Hyperkeratosis; Keratosis Palmaris Et Plantaris; Keratin, type I cytoskeletal 10
UniProt
P02535
Reactivity
Mouse
Field of Research
Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.12 μg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KRT10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KRT10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KRT10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KRT10 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 μg/mL

UniProtKB · P02535

Keratin, type I cytoskeletal 10

K1C10_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P02535
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Krt10
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
P08731, Q8BUX3, Q8BV09, Q8BVU3, Q9CXH6, Q9JKB4
Protein keywords

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filamentKeratinSecreted

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondMethylationPhosphoprotein

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