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Mouse THBS4 (Thrombospondin 4) Microsample ELISA Kit

CAT: 0013-GTR18873441-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873441-01Size:48 Tests
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Description
Mouse THBS4 (Thrombospondin 4) Microsample ELISA Kit
Product Name Alternative
TSP4
UniProt
Q9Z1T2
Reactivity
Mouse
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
9.21 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse THBS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse THBS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse THBS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse THBS4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000 ng/mL

UniProtKB · Q9Z1T2

Thrombospondin-4

TSP4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9Z1T2
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Thbs4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q9QYS3, Q9WUE0
Protein keywords

Ligand

Calcium

Biological process

Cell adhesionTissue remodelingUnfolded protein response

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignal

Cellular component

Endoplasmic reticulumExtracellular matrixSarcoplasmic reticulumSecreted

Molecular function

Growth factorMitogen

Technical term

Reference proteome

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