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Human THBS2 (Thrombospondin 2) Microsample ELISA Kit

CAT: 0013-GTR18873227-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873227-01Size:48 Tests
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Description
Human THBS2 (Thrombospondin 2) Microsample ELISA Kit
Product Name Alternative
TSP2
UniProt
P35442
Reactivity
Human
Field of Research
Cancer Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human THBS2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human THBS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human THBS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human THBS2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.47-30 ng/mL

UniProtKB · P35442

Thrombospondin-2

TSP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35442
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
THBS2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A6H8N1, A7E232, Q5RI52
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Ligand

Calcium

Biological process

Cell adhesion

Disease

Disease variantEhlers-Danlos syndrome

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignal

Molecular function

Heparin-binding

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