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Mouse bTC (Betacellulin) Microsample ELISA Kit

CAT: 0013-GTR18873248-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873248-01Size:48 Tests
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Description
Mouse bTC (Betacellulin) Microsample ELISA Kit
Product Name Alternative
Probetacellulin
UniProt
Q05928
Reactivity
Mouse
Field of Research
Cancer Biology, Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
6.5 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bTC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bTC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse bTC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bTC in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

UniProtKB · Q05928

Probetacellulin

BTC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q05928
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Btc
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembraneSecreted

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane helix

Molecular function

Growth factorMitogen

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