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Human GDF5 (Growth Differentiation Factor 5) Microsample ELISA Kit

CAT: 0013-GTR18873229-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873229-01Size:48 Tests
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Description
Human GDF5 (Growth Differentiation Factor 5) Microsample ELISA Kit
Product Name Alternative
CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4
UniProt
P43026
Reactivity
Human
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
32 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000 pg/mL

UniProtKB · P43026

Growth/differentiation factor 5

GDF5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43026
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GDF5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GDF-5
EC number
—
Processing
Precursor
Secondary accessions
E1P5Q2, Q96SB1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneMembraneSecreted

Biological process

Chondrogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineGrowth factor

Disease

Disease variantDwarfism

Domain

Signal

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