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Mouse GZMA (Granzyme A) Microsample ELISA Kit

CAT: 0013-GTR18873174-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873174-01Size:48 Tests
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Description
Mouse GZMA (Granzyme A) Microsample ELISA Kit
Product Name Alternative
CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1
UniProt
P11032
Reactivity
Mouse
Field of Research
Immunology & Inflammation, Infectious Disease & Virology, Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.057 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GZMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GZMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GZMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GZMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P11032

Granzyme A

GRAA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P11032
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gzma
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.78
Processing
Precursor
Secondary accessions
P15118
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cytolysis

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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