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Mouse GZMB (Granzyme B) Microsample ELISA Kit

CAT: 0013-GTR18873171-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873171-01Size:48 Tests
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Description
Mouse GZMB (Granzyme B) Microsample ELISA Kit
Product Name Alternative
GZM-B; HLP; CTLA1; CCPI; CGL1; CSP-B; CSPB; CTSGL1; SECT; Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Fragmentin 2; Cytotoxic Serine Protease B
UniProt
P04187
Reactivity
Mouse
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
13.81 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GZMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GZMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GZMB in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000 ng/mL

UniProtKB · P04187

Granzyme B(G,H)

GRAB_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P04187
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gzmb
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.79
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

ApoptosisCytolysis

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

LysosomeSecreted

Domain

Signal

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