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Human GZMB (Granzyme B) Microsample ELISA Kit

CAT: 0013-GTR18873173-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873173-01Size:48 Tests
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Description
Human GZMB (Granzyme B) Microsample ELISA Kit
Product Name Alternative
GZM-B; HLP; CTLA1; CCPI; CGL1; CSP-B; CSPB; CTSGL1; SECT; Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Fragmentin 2; Cytotoxic Serine Protease B
UniProt
P10144
Reactivity
Human
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
6.4 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMB in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P10144

Granzyme B

GRAB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10144
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GZMB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.79
Processing
Precursor
Secondary accessions
Q8N1D2, Q9UCC1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

ApoptosisCytolysis

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

LysosomeSecreted

Domain

Signal

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