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Human CX40 (Connexin 40) Microsample ELISA Kit

CAT: 0013-GTR18873091-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873091-01Size:48 Tests
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Description
Human CX40 (Connexin 40) Microsample ELISA Kit
Product Name Alternative
GJA5; GJ-A5; Gap Junction Protein Alpha 5
UniProt
P36382
Reactivity
Human
Field of Research
Cardiovascular Research, Infectious Disease & Virology, Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.121 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CX40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CX40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CX40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CX40 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P36382

Gap junction alpha-5 protein

CXA5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P36382
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GJA5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q5T3B6, Q5U0N6
Protein keywords

Disease

Atrial fibrillationCardiomyopathyDisease variant

Cellular component

Cell junctionCell membraneGap junctionMembrane

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

Domain

TransmembraneTransmembrane helix

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