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Human AIRE (Autoimmune Regulator) Microsample ELISA Kit

CAT: 0013-GTR18872697-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872697-01Size:48 Tests
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Description
Human AIRE (Autoimmune Regulator) Microsample ELISA Kit
Product Name Alternative
AIRE1; APECED; APS1; APSI; PGA1; Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy
UniProt
O43918
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.055 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIRE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIRE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIRE in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · O43918

Autoimmune regulator

AIRE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43918
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AIRE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2RP50, O43922, O43932, O75745
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmNucleus

Disease

Disease variant

Ligand

Metal-bindingZinc

PTM

Phosphoprotein

Domain

RepeatZinc-finger

Biological process

TranscriptionTranscription regulation

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