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Mouse F12 (Coagulation Factor XII) Microsample ELISA Kit

CAT: 0013-GTR18872693-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872693-01Size:48 Tests
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Description
Mouse F12 (Coagulation Factor XII) Microsample ELISA Kit
Product Name Alternative
FXII; HAF; Contact Factor; Hageman Factor
UniProt
Q80YC5
Reactivity
Mouse
Field of Research
Cardiovascular Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma
Sensitivity
26 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
62.5-4000 pg/mL

UniProtKB · Q80YC5

Coagulation factor XII

FA12_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q80YC5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F12
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.38
Processing
Precursor
Secondary accessions
O35727, Q6PER0
Protein keywords

Biological process

Blood coagulationFibrinolysisHemostasis

PTM

Disulfide bondGlycoproteinZymogen

Domain

EGF-like domainKringleRepeatSignal

Molecular function

HydrolaseProteaseSerine protease

Technical term

Reference proteome

Cellular component

Secreted

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