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Human PLVAP (Plasmalemma Vesicle Associated Protein) Microsample ELISA Kit

CAT: 0013-GTR18872670-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872670-01Size:48 Tests
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Description
Human PLVAP (Plasmalemma Vesicle Associated Protein) Microsample ELISA Kit
Product Name Alternative
FELS; PV-1; PV1; gp68; Fenestrated-Endothelial Linked Structure Protein; Plasmalemma vesicle protein 1
UniProt
Q9BX97
Reactivity
Human
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.058 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLVAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLVAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLVAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLVAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9BX97

Plasmalemma vesicle-associated protein

PLVAP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BX97
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PLVAP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q86VP0, Q8N8Y0, Q8ND68, Q8TER8, Q9BZD5
Protein keywords

Cellular component

Cell membraneCytoplasmMembrane

Domain

Coiled coilSignal-anchorTransmembraneTransmembrane helix

Disease

Disease variant

PTM

Glycoprotein

Technical term

Proteomics identificationReference proteome

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