Human GAP43 (Growth Associated Protein 43) Microsample ELISA Kit

• Product Name Alternative:

  F1; B-50; PP46; Neuron Growth-Associated Protein 43; Neuromodulin; Axonal Membrane Protein GAP-43; Protein F1; Calmodulin-Binding Protein P-57; Neural Phosphoprotein B-50

• UniProt:

  P17677

• Reactivity:

  Human

• Field of Research:

  Neuro science; Developmental science

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAP43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAP43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAP43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAP43 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5h

• Standard:

  10 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.16-10 ng/mL

• Sensitivity:

  0.06 ng/mL