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Mouse CHGA (Chromogranin A) Microsample ELISA Kit

CAT: 0013-GTR18872480-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872480-01Size:48 Tests
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Description
Mouse CHGA (Chromogranin A) Microsample ELISA Kit
Product Name Alternative
CGA; SP-I; Parathyroid Secretory Protein 1; Vasostatin; Pancreastatin; Parastatin; Pituitary secretory protein I
UniProt
P26339
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.052 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHGA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHGA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHGA in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P26339

Chromogranin-A

CMGA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P26339
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Chga
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
CgA
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

PTM

AmidationCleavage on pair of basic residuesDisulfide bondGlycoproteinOxidationPhosphoproteinProteoglycanPyrrolidone carboxylic acid

Ligand

Calcium

Cellular component

Cytoplasmic vesicleSecreted

Domain

Signal

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