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Human ACTN3 (Actinin Alpha 3) Microsample ELISA Kit

CAT: 0013-GTR18872461-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872461-01Size:48 Tests
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Description
Human ACTN3 (Actinin Alpha 3) Microsample ELISA Kit
Product Name Alternative
Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
UniProt
Q08043
Reactivity
Human
Field of Research
Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.63 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
1.57-100 ng/mL

UniProtKB · Q08043

Alpha-actinin-3

ACTN3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q08043
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTN3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A6NP77, Q4KKV2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Acetylation

Molecular function

Actin-binding

Ligand

CalciumMetal-binding

Domain

Repeat

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