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Human ACTN2 (Actinin Alpha 2) Microsample ELISA Kit

CAT: 0013-GTR18872876-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872876-01Size:48 Tests
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Description
Human ACTN2 (Actinin Alpha 2) Microsample ELISA Kit
Product Name Alternative
Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein
UniProt
P35609
Reactivity
Human
Field of Research
Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.127 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P35609

Alpha-actinin-2

ACTN2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35609
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTN2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B1ANE4, B2RCS5, Q86TF4, Q86TI8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-binding

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Disease

CardiomyopathyDisease variant

Cellular component

Cytoplasm

PTM

PhosphoproteinUbl conjugation

Domain

Repeat

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