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Human 5HTR2A (5-Hydroxytryptamine Receptor 2A) Microsample ELISA Kit

CAT: 0013-GTR18872022-01Size: 48 TestsDry Ice: NoHazardous: No
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Description
Human 5HTR2A (5-Hydroxytryptamine Receptor 2A) Microsample ELISA Kit
Product Name Alternative
5-HT2A; HTR2; Serotonin Receptor 2A
UniProt
P28223
Reactivity
Human
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.069 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR2A in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P28223

5-hydroxytryptamine receptor 2A

5HT2A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28223
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HTR2A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
5-HT-2, 5-HT-2A
EC number
—
Processing
—
Secondary accessions
B2RAC5, B4DZ79, F5GWE8, Q5T8C0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

BehaviorHost-virus interaction

Cellular component

Cell membraneCell projectionCytoplasmic vesicleMembraneSynapse

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

G protein-coupled receptorHost cell receptor for virus entryReceptorTransducer

Domain

TransmembraneTransmembrane helix

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