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Human 5HTR2B (5-Hydroxytryptamine Receptor 2B) Microsample ELISA Kit

CAT: 0013-GTR18871835-01Size: 48 TestsDry Ice: NoHazardous: No
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Description
Human 5HTR2B (5-Hydroxytryptamine Receptor 2B) Microsample ELISA Kit
Product Name Alternative
5HTR2B; 5HTR2-B; 5-HTR2-B
UniProt
P41595
Reactivity
Human
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.119 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR2B in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P41595

5-hydroxytryptamine receptor 2B

5HT2B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41595
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HTR2B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
5-HT-2B, 5-HT2B
EC number
—
Processing
—
Secondary accessions
B2R9D5, Q53TI1, Q62221, Q6P523
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Behavior

Cellular component

Cell membraneMembraneSynapseSynaptosome

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Molecular function

G protein-coupled receptorReceptorTransducer

Domain

TransmembraneTransmembrane helix

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