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Human CNX (Calnexin) Microsample ELISA Kit

CAT: 0013-GTR18871995-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871995-01Size:48 Tests
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Description
Human CNX (Calnexin) Microsample ELISA Kit
Product Name Alternative
CANX; CNX; IP90; P90; Major Histocompatibility Complex Class I Antigen-Binding Protein p88
UniProt
P27824
Reactivity
Human
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.065 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CNX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CNX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CNX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CNX in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P27824

Calnexin

CALX_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P27824
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CANX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3
Protein keywords

PTM

AcetylationDisulfide bondLipoproteinPalmitatePhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

CalciumLectinMetal-binding

Molecular function

Chaperone

Technical term

Direct protein sequencingProteomics identificationReference proteome

Cellular component

Endoplasmic reticulumMembraneMitochondrion

Biological process

Host-virus interaction

Domain

RepeatSignalTransmembraneTransmembrane helix

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