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Human N4BP2 (NEDD4 Binding Protein 2) Microsample ELISA Kit

CAT: 0013-GTR18871888-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871888-01Size:48 Tests
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Description
Human N4BP2 (NEDD4 Binding Protein 2) Microsample ELISA Kit
Product Name Alternative
B3BP; BCL3 Binding Protein
UniProt
Q86UW6
Reactivity
Human
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.128 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N4BP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N4BP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N4BP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N4BP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · Q86UW6

NEDD4-binding protein 2

N4BP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q86UW6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
N4BP2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
N4BP2
EC number
3.-.-.-
Processing
—
Secondary accessions
A0AVR3, Q9NVK2, Q9P2D4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Domain

Coiled coil

Cellular component

Cytoplasm

Molecular function

Hydrolase

PTM

PhosphoproteinUbl conjugation

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