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Rat NCAN (Neurocan) Microsample ELISA Kit

CAT: 0013-GTR18871647-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871647-01Size:48 Tests
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Description
Rat NCAN (Neurocan) Microsample ELISA Kit
Product Name Alternative
CSPG3; NEUR; Neurocan Core Protein; Chondroitin Sulfate Proteoglycan 3; Neurocan Proteoglycan
UniProt
P55067
Reactivity
Rat
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.05 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NCAN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NCAN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NCAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NCAN in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P55067

Neurocan core protein

NCAN_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P55067
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ncan
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumHyaluronic acidLectin

Biological process

Cell adhesion

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinProteoglycan

Domain

EGF-like domainImmunoglobulin domainRepeatSignalSushi

Cellular component

Secreted

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