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Rat IREB2 (Iron Responsive Element Binding Protein 2) Microsample ELISA Kit

CAT: 0013-GTR18871643-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871643-01Size:48 Tests
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Description
Rat IREB2 (Iron Responsive Element Binding Protein 2) Microsample ELISA Kit
Product Name Alternative
IRP2; Iron regulatory protein 2
UniProt
Q62751
Reactivity
Rat
Field of Research
Neuroscience; Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.105 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IREB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IREB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IREB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IREB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · Q62751

Iron-responsive element-binding protein 2

IREB2_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q62751
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ireb2
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
IRE-BP 2
EC number
—
Processing
—
Secondary accessions
Q66HL4
Protein keywords

Ligand

4Fe-4SIronIron-sulfurMetal-binding

Cellular component

Cytoplasm

Technical term

Reference proteome

Molecular function

RNA-binding

PTM

Ubl conjugation

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