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Mouse CTSL (Cathepsin L) Microsample ELISA Kit

CAT: 0013-GTR18873532-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873532-01Size:48 Tests
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Description
Mouse CTSL (Cathepsin L) Microsample ELISA Kit
Product Name Alternative
CTSL1; CATL; CTS-L; MEP; Cathepsin L1; Major excreted protein
UniProt
P06797
Reactivity
Mouse
Field of Research
Cancer Biology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.061 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CTSL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CTSL in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P06797

Procathepsin L

CATL1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06797
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ctsl
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.22.15
Processing
Precursor
Secondary accessions
Q91UZ0
Protein keywords

Cellular component

Cell membraneCytoplasmic vesicleLysosomeMembraneSecreted

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseThiol protease

Ligand

Metal-bindingZinc

Domain

Signal

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