Framycetin

• Description:

  Framycetin (Neomycin B), an aminoglycoside antibiotic, is a potent RNase P cleavage activity inhibitor with a Ki of 35 μM. Framycetin competes for specific divalent metal ion binding sites in RNase P RNA. Framycetin inhibits hammerhead ribozyme with a Ki of 13.5 μM. Framycetin, a 5″-azido neomycin B precursor, binds the Drosha site in miR-525 and is used for hepatic encephalopathy and enteropathogenic E. coli infections[1][2].

• Product Name Alternative:

  Neomycin B; Fradiomycin B

• UNSPSC:

  12352005

• Hazard Statement:

  H317, H334

• Target:

  Antibiotic; Bacterial

• Type:

  Reference compound

• Related Pathways:

  Anti-infection

• Applications:

  COVID-19-immunoregulation

• Field of Research:

  Infection

• Assay Protocol:

  https://www.medchemexpress.com/Framycetin.html

• Concentration:

  16.27 mM * 2.5 mL in 0.9% NaCl

• Solubility:

  DMSO : 50 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)

• Smiles:

  N[C@@H](C[C@H]1N)[C@@]([C@@H]([C@H]1O)O[C@@](O[C@H](CO)[C@H]2O[C@@]([C@@H]([C@@H](O)[C@@H]3O)N)([H])O[C@H]3CN)([H])[C@@H]2O)([H])O[C@H]([C@@H]([C@@H](O)[C@@H]4O)N)O[C@@H]4CN

• Molecular Formula:

  C23H46N6O13

• Molecular Weight:

  614.64

• Precautions:

  H317, H334

• References & Citations:

  [1]N E Mikkelsen, et al. Inhibition of RNase P RNA Cleavage by Aminoglycosides. Proc Natl Acad Sci U S A. 1999 May 25;96 (11) :6155-60.|[2]Childs-Disney JL, et al. Small Molecule Targeting of a MicroRNA Associated with Hepatocellular Carcinoma. ACS Chem Biol. 2016 Feb 19;11 (2) :375-80.|[3]Stampfl S, et al. Monovalent ion dependence of neomycin B binding to an RNA aptamer characterized by spectroscopic methods. Chembiochem. 2007 Jul 9;8 (10) :1137-45.|[4]Hoch I, et al. Antibiotic inhibition of RNA catalysis: neomycin B binds to the catalytic core of the td group I intron displacing essential metal ions. J Mol Biol. 1998 Sep 25;282 (3) :557-69.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Solution, -20°C, 2 years

• Scientific Category:

  Reference compound1

• Clinical Information:

  Launched

• Isoform:

  Aminoglycoside

• CAS Number:

  119-04-0