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Human TRY (Trypsin) Microsample ELISA Kit

CAT: 0013-GTR18871504-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871504-01Size:48 Tests
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Description
Human TRY (Trypsin) Microsample ELISA Kit
Product Name Alternative
Trypsin
Reactivity
Human
Field of Research
Molecular Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.067 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRY in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

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