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Pig Trypsin (TRY) ELISA Kit

CAT: 0013-GTR18811628-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18811628-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trypsin (TRY) .
Product Name Alternative
TRY
UniProt
P00761
Reactivity
Porcine
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.64 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TRY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TRY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TRY in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL

UniProtKB · P00761

Trypsin

TRYP_PIG · Sus scrofa

View on UniProt ↗
Primary accession
P00761
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Sus scrofa (Pig)
Taxonomy ID
9823
Alternative names
—
EC number
3.4.21.4
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Ligand

CalciumMetal-binding

Biological process

Digestion

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

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