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Mouse MFAP5 (Microfibrillar Associated Protein 5) Microsample ELISA Kit

CAT: 0013-GTR18871406-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871406-01Size:48 Tests
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Description
Mouse MFAP5 (Microfibrillar Associated Protein 5) Microsample ELISA Kit
Product Name Alternative
MAGP2; MP25; Microfibril-associated glycoprotein 2
UniProt
Q9QZJ6
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.067 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MFAP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MFAP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MFAP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MFAP5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9QZJ6

Microfibrillar-associated protein 5

MFAP5_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9QZJ6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mfap5
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
MFAP-5
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Disulfide bondGlycoprotein

Cellular component

Extracellular matrixSecreted

Technical term

Reference proteome

Domain

Signal

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